|
KMID : 0613820120220121614
|
|
Journal of Life Science
2012 Volume.22 No. 12 p.1614 ~ p.1620
|
|
|
Transgenic Rice Expressing Snowdrop Lectin (Galanthus nivalis agglutinin; GNA) Shows Resistance to Rice Brown Planthopper (Nilaparvata lugens Stal)
|
|
Lee Soo-In
Yoon In-Sun
Kim Jin-A
Hong Joon-Ki
Park Beom-Seok
Lee Yeon-Hee
|
|
|
Abstract
|
|
|
|
Transgenic rice plants with increased resistance to rice brown planthopper (Nilaparvata lugens St${\aa}$ 수식 이미지l) were generated by particle bombardment-mediated transformation of plants with a gene encoding snowdrop lectin (Galanthus nivalis agglutinin; GNA) under control of the rice Rubisco small subunit (rbcS) promoter.. A large number of transgenic rice plants containing the GNA gene were generated. The integration, expression, and inheritance of this gene in the R₁ and R₂ generations were demonstrated by Southern and western blot analyses. The plants contained one to five copies of the transgene. The GNA protein comprised approximately 0.01-2.0% of total soluble protein in the R₁ and R₂ transgenic plants. Insect bioassays and feeding studies showed that the GNA protein expressed in the R₂ transgenic rice plants reduced the survival of brown planthoppers. The introduction of GNA into rice plants therefore can help to control insect pests.
|
|
|
KEYWORD
|
|
|
Brown planthopper, Galanthus nivalis agglutinin (GNA), insect resistance, particle bombardment, transgenic rice plants
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|